Movies & Images
Research Unit for
Molecular and Cellular Physiology
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Feel free to view our small image & movie library:
*For further technical information please visit our Technique site
| Image description | Image* | Movie description | Movie* |
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| #I-1: 3D reconstruction of mitochondria in living human endothelial cells that were transiently tansfected with ratiometric-pericam-mt (a kind gift from Prof. Atsuhi Miyawaki, Japan). |  |
#M-1: This is the way how we position the patch pipette on cell membrane that is close to superficial intracellular organelles. Mostly we apply our Physiological Patch technique (Malli et al). |  |
| #I-2: 3D reconstruction of mitochondria in living human endothelial cells that were transiently tansfected with mitochondria-targeted DsRed 2 (a kind gift from Prof. Tullio Pozzan, Italy) and ApoK1er (Osibow et al). |  |
#M-2: Endothellial cells were transiently transfected with cytosolic GFP and mitochondria-targeted DsRed. Cytosolic surface and mitochondrial structure are calculated by surface- and volume-rendering, respectively. |
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| #I-3: 3D reconstruction of the ER in living human endothelial cells that were transiently transfected with Cam4er (a kind gift from Profs. Roger Y. Tsien and Atsuhi Miyawaki) |  |
#M-3: 4D movie on the fragmentation of mitochondrial network in endothelial cells upon exposure to superoxide anions. The time frame is overall 1h and grid distance indicates 1 µm. |  |
| #I-4: High D-glucose but not high mannitol yield fragmentation of mitochondria within 2 h of incubation. Three-dimensional architecture of the mitochondrial network was monitored using an ACLSM using a 100x objective (NA 1.45) and a z-step size of 100 nm. After image deconvolution 3D restoration and surface rendering were performed using Bitplane Imaris 3.3. |  |
#M-4: Spike-like accumulation of PKCalpha in endothelial cells upon cell stimulation under conditions of distrubed intracellular Ca2+ cycling. Endothelial cells expressing PKCalpha-GFP were stimulated with BHQ & histamine and the accumulation of PKCalpha was monitored using the ACLSM. |  |
| #M-5: Mitochondrial movements are sensitive to cytosolic Ca2+ elevation. Human endothelial cells were transiently transfected with ratiometric-pericam-mt and stimulated with histamine as indicated. |  |
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